We present a microfluidic platform developed by Bflow designed to create a in vitro Blood-Brain Barrier (BBB) model for drug screening and neuroscience research. The chip, fabricated in biocompatible PDMS using a scalable process based on 3D micro-printed resin templates, incorporates a three-channel architecture: a wide channel for culturing endothelial cells under physiological shear stress, a central narrow channel for extracellular matrix (ECM) gel, and a third wider channel for brain cell co-culture. Crucially, the system features two independently perfused channels and an optimized circular channel cross-section, which ensures homogeneous fluid shear stress and allows for the use of distinct culture media, a significant advantage over conventional Transwell and rectangular-channel systems. This Bflow microfluidic chip provides a dynamic and physiologically relevant system, advancing the field beyond static models to accurately measure barrier permeability, cellular responses and neurotoxicity.
